Microbe Investigations AG
LS24-03459

Report Date: 28 October, 2024
Producer: Interstream d.o.o
Brand: STREAM

Index:

• Test overview and summary
• Application data
• Microbiology: Antiviral Activity
• Annexes to the test report

Test Report Overview

General Info

• Producer: Interstream d.o.o
• Brand owner: Interstream d.o.o
• Brand label: STREAM
• Reason for testing: Customer Quality Validation
• Application by: Not specified
• Effects: Scale 3 - Bulk trial

Test Methods Carried Out in This Report

Effect / Property	Testing Standard	Test Parameter
Quantitative virucidal suspension test for Hand Sanitizer and Disinfectants	EN 14476	Poliovirus (Fridrich Loeffler Institut (RVB-1260)

Test Summary / Comments:

• In the test EN 14476, the sample "Disinfectant STREAM" when challenged for a contact time of 30 min at 15°C, showed a 4.6 log (99.997%) reduction against Poliovirus.

Samples Information

Sample	Sample Description
1	Disinfectant STREAM

Test Sample Preparation:

1. Mix 10 ml of liquid component with 0.52 g of solid component.
2. Mixed components were left together for 3 hours before use.
3. Test products for testing were prepared by adding 30 ml of mixed components in 1L of water.

Microbiology: Antiviral Activity

EN 14476 – Poliovirus (Fridrich Loeffler Institut (RVB-1260))

Parameter	Value
Infectivity titre test	TCID50 method
Test sample concentration	80%
Test conditions	Clean condition
Contact time (min)	30
Virus control (Log10 TCID50)	7.33
Test sample (Log10 TCID50)	2.73
Log reduction (Log10 TCID50)	4.6
Percent reduction (%)	99.997%
Virucidal activity	Pass

EN 14476:2019-10 – Quantitative Virucidal Suspension Test for Disinfectant

Test Details:

• Test Organism: Poliovirus (Fridrich Loeffler Institut (RVB-1260))
• Test Specimen: Disinfectant STREAM (2023:2380)
• Test Inoculum: 1 part of test virus into 9 parts of test mixture (8 parts test specimen + 1 part of interfering substance)
• Contact Time: 30 min
• Test Temperature: 15°C ± 1°C

Outline of Test Method

• 1 part of test virus (Poliovirus) was inoculated separately into 9 parts of control (hard water + interfering substance) specimen and 9 parts of test (Disinfectant + interfering substance).
• The above mixture was pulse vortexed and incubated at a specified temperature (15°C ± 1°C) for 30 min.
• After the specified contact time (30 min), 0.5 ml of the mixture was added to 4.5 ml of ice-cold medium for neutralization of disinfectant.
• The neutralized suspension was subjected to serial dilution (1:10) using maintenance medium.
• Virus recovery from the control and test sample was done by adding the serially diluted sample to cells in a 96-well plate for TCID50 determination.

Antiviral Activity (R) Calculation

Formula:
R = a - b

Where:

• R = Log reduction
• a = Log TCID50 of Virus control titration
• b = Log TCID50 of Test sample virus titration

Percent Reduction Calculation

Formula:
Percent Reduction = 100 * (1 – 10^R)

Log Reduction (RT)	Percent Reduction
4	99.99%
3	99.9%
2	99%
1	90%

• Log reduction should be at least 4 log for the sample to pass.

Testing Procedure (EN 14476)

This includes:

1. Sample Preparation (test tube A)
2. Inoculation (adding test virus suspension)
3. Incubation (30 min at specified temperature)
4. Neutralization (using cold medium)
5. Serial dilution and processing
6. Virus titration using TCID50 method
7. Data analysis and reduction calculation

Company Information

Microbe Investigations AG | Enzenried 12 | 8008 Zürich | Switzerland
Phone: +41 76 380 48 35