EU Standart EN 1276. Test Pseudomonas aeruginosa
Microbe Investigations AG
LS24-01852
Report date: May 28, 2024
Interstream d.o.o
Brand: STREAM
Index
- Test overview and summary
- Application data
- Test details
- Annexes to the test report
Test report overview
General Info:
- Interstream d.o.o
- Brand owner: STREAM
- Contact: D. Karachun
- Reason for testing: Customer Quality Validation (CQV)
- Application by: Not specified
- Effects: Scale 3 - Bulk trial
Test methods carried out in this report:
EN 1276:2019
"Chemical disinfectants and antiseptics - Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas."
Test summary/comments:
In the "Test EN 1276:2019", the sample "2023-2380 Disinfectant STREAM" when challenged with Pseudomonas aeruginosa (ATCC-15442) for a contact time of 30 minutes showed 5.046 log reduction (99.9999% antimicrobial activity).
Samples information
|
Sample |
Sample Description |
|
1 |
2023-2380 Disinfectant STREAM |
Test Sample Preparation:
- Mix 5L of liquid component with 0.26 kg of solid component (maintaining the same ratio in smaller batches).
- Mixed components were left together for 3 hours before use.
- Test products for testing were prepared by adding 1ml of mixed components to 3L of water.
Test Details:
- Test organism: Pseudomonas aeruginosa
- Contact time: 30 minutes
- Test condition: Clean condition
- Test temperature: 15°C
Annexure
- Test organism: Pseudomonas aeruginosa
- Test temperature: 15°C
- Incubation temperature: 36°C ± 1°C
- Dilution-neutralization method: Spread plate
- Numbers of plates: 2/ml
- Neutralizer: B
- Test conditions: Clean conditions
Validation and control:
|
Validation Suspension (Nv) |
Control (A) |
Control (B) |
Method Validation (C) |
|
Vc1: 132 |
Vc1: 141 |
Vc1: 143 |
Vc1: 111 |
|
Vc2: 176 |
Vc2: 123 |
Vc2: 155 |
Vc2: 131 |
Test suspension (N and No):
- N: 191.5×10 6
- lg N = 8.281
- lg No = 7.281
Test Results:
|
Conc. of the Product |
Contact Time |
Dilution Step |
Vc1 |
Vc2 |
Na (×10 or wm ×10) |
lg Na |
lg R (lg No - lg Na) |
|
1ml stock diluted in 3L water |
5 min |
10° |
156 |
189 |
172.5 |
2.235 |
5.046 |
Antimicrobial activity: 99.999%
Company Information:
Microbe Investigations AG | Enzenried 12 | 8008 Zürich | Switzerland
Phone: +41 76 380 48 35
